Review





Similar Products

t175 tissue culture flasks  (ATCC)
92
ATCC t175 tissue culture flasks
T175 Tissue Culture Flasks, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t175 tissue culture flasks/product/ATCC
Average 92 stars, based on 1 article reviews
t175 tissue culture flasks - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
human recombinant mip 1α protein  (Sino Biological)
94
Sino Biological human recombinant mip 1α protein
Human Recombinant Mip 1α Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human recombinant mip 1α protein/product/Sino Biological
Average 94 stars, based on 1 article reviews
human recombinant mip 1α protein - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
human ccl3 / mip1a protein  (Sino Biological)
94
Sino Biological human ccl3 / mip1a protein
Human Ccl3 / Mip1a Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human ccl3 / mip1a protein/product/Sino Biological
Average 94 stars, based on 1 article reviews
human ccl3 / mip1a protein - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
ccl3  (Sino Biological)
94
Sino Biological ccl3
CD177 promotes T‐cell transendothelial migration. (a) Human colorectal cancer (CRC) specimens were stained with DAPI (blue), PEACM‐1 (green), CD4 (white), FOXP3 (red) and CD177 (yellow), respectively. The scale bar (50 μm) is placed at the left corner of each image. (b) CD177 + Treg cells in peripheral blood mononuclear cells from CRC patients ( n = 12) and healthy volunteers ( n = 6). (c) Diagram of CD177‐EGFP in pHR plasmid. (d) Model of iTreg cells induction, transfection, selection and then expanded for 2 days before the assay. (e) Diagram of the transendothelial migration assay. HUVECs were seeded on the upper surface of the transwell insert and iTreg cells were placed in the upper chamber filled with X‐VIVO. The lower chamber was filled with X‐VIVO containing CCL19 and CCL22 (200 ng mL −1 ). (f) Migration rate of OE iTreg cells and EV iTreg cells after 4 h. OE: CD177‐overexpression; EV: empty vector control. (g) Migration rate of OE iTreg cells at 4 h under different treatments. NC, negative control; α177, anti‐CD177; αPE, anti‐PECAM‐1. (h) Migration rate of EV iTreg cells after 4 h under different treatments. (i) PD‐1 expression of OE iTreg cells and EV iTreg cells. (j) Migration rate of OE CD8 + T cells and EV CD8 + T cells at 4 h. The lower chamber was filled with X‐VIVO containing <t>CCL3</t> (200 ng mL −1 ) in (j) . Data are represented as mean ± SEM and were analysed by the unpaired Student's t‐ test (b, f, i, j) or one‐way ANOVA (g, h) . * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are pooled from three independent experiments.
Ccl3, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ccl3/product/Sino Biological
Average 94 stars, based on 1 article reviews
ccl3 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
t75  (ATCC)
92
ATCC t75
CD177 promotes T‐cell transendothelial migration. (a) Human colorectal cancer (CRC) specimens were stained with DAPI (blue), PEACM‐1 (green), CD4 (white), FOXP3 (red) and CD177 (yellow), respectively. The scale bar (50 μm) is placed at the left corner of each image. (b) CD177 + Treg cells in peripheral blood mononuclear cells from CRC patients ( n = 12) and healthy volunteers ( n = 6). (c) Diagram of CD177‐EGFP in pHR plasmid. (d) Model of iTreg cells induction, transfection, selection and then expanded for 2 days before the assay. (e) Diagram of the transendothelial migration assay. HUVECs were seeded on the upper surface of the transwell insert and iTreg cells were placed in the upper chamber filled with X‐VIVO. The lower chamber was filled with X‐VIVO containing CCL19 and CCL22 (200 ng mL −1 ). (f) Migration rate of OE iTreg cells and EV iTreg cells after 4 h. OE: CD177‐overexpression; EV: empty vector control. (g) Migration rate of OE iTreg cells at 4 h under different treatments. NC, negative control; α177, anti‐CD177; αPE, anti‐PECAM‐1. (h) Migration rate of EV iTreg cells after 4 h under different treatments. (i) PD‐1 expression of OE iTreg cells and EV iTreg cells. (j) Migration rate of OE CD8 + T cells and EV CD8 + T cells at 4 h. The lower chamber was filled with X‐VIVO containing <t>CCL3</t> (200 ng mL −1 ) in (j) . Data are represented as mean ± SEM and were analysed by the unpaired Student's t‐ test (b, f, i, j) or one‐way ANOVA (g, h) . * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are pooled from three independent experiments.
T75, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t75/product/ATCC
Average 92 stars, based on 1 article reviews
t75 - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
r strictum dsm 11289 t  (DSMZ)
91
DSMZ r strictum dsm 11289 t
CD177 promotes T‐cell transendothelial migration. (a) Human colorectal cancer (CRC) specimens were stained with DAPI (blue), PEACM‐1 (green), CD4 (white), FOXP3 (red) and CD177 (yellow), respectively. The scale bar (50 μm) is placed at the left corner of each image. (b) CD177 + Treg cells in peripheral blood mononuclear cells from CRC patients ( n = 12) and healthy volunteers ( n = 6). (c) Diagram of CD177‐EGFP in pHR plasmid. (d) Model of iTreg cells induction, transfection, selection and then expanded for 2 days before the assay. (e) Diagram of the transendothelial migration assay. HUVECs were seeded on the upper surface of the transwell insert and iTreg cells were placed in the upper chamber filled with X‐VIVO. The lower chamber was filled with X‐VIVO containing CCL19 and CCL22 (200 ng mL −1 ). (f) Migration rate of OE iTreg cells and EV iTreg cells after 4 h. OE: CD177‐overexpression; EV: empty vector control. (g) Migration rate of OE iTreg cells at 4 h under different treatments. NC, negative control; α177, anti‐CD177; αPE, anti‐PECAM‐1. (h) Migration rate of EV iTreg cells after 4 h under different treatments. (i) PD‐1 expression of OE iTreg cells and EV iTreg cells. (j) Migration rate of OE CD8 + T cells and EV CD8 + T cells at 4 h. The lower chamber was filled with X‐VIVO containing <t>CCL3</t> (200 ng mL −1 ) in (j) . Data are represented as mean ± SEM and were analysed by the unpaired Student's t‐ test (b, f, i, j) or one‐way ANOVA (g, h) . * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are pooled from three independent experiments.
R Strictum Dsm 11289 T, supplied by DSMZ, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r strictum dsm 11289 t/product/DSMZ
Average 91 stars, based on 1 article reviews
r strictum dsm 11289 t - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier
nci-11292 human nsclc xenograft model  (Jackson Laboratory)
90
Jackson Laboratory nci-11292 human nsclc xenograft model
CD177 promotes T‐cell transendothelial migration. (a) Human colorectal cancer (CRC) specimens were stained with DAPI (blue), PEACM‐1 (green), CD4 (white), FOXP3 (red) and CD177 (yellow), respectively. The scale bar (50 μm) is placed at the left corner of each image. (b) CD177 + Treg cells in peripheral blood mononuclear cells from CRC patients ( n = 12) and healthy volunteers ( n = 6). (c) Diagram of CD177‐EGFP in pHR plasmid. (d) Model of iTreg cells induction, transfection, selection and then expanded for 2 days before the assay. (e) Diagram of the transendothelial migration assay. HUVECs were seeded on the upper surface of the transwell insert and iTreg cells were placed in the upper chamber filled with X‐VIVO. The lower chamber was filled with X‐VIVO containing CCL19 and CCL22 (200 ng mL −1 ). (f) Migration rate of OE iTreg cells and EV iTreg cells after 4 h. OE: CD177‐overexpression; EV: empty vector control. (g) Migration rate of OE iTreg cells at 4 h under different treatments. NC, negative control; α177, anti‐CD177; αPE, anti‐PECAM‐1. (h) Migration rate of EV iTreg cells after 4 h under different treatments. (i) PD‐1 expression of OE iTreg cells and EV iTreg cells. (j) Migration rate of OE CD8 + T cells and EV CD8 + T cells at 4 h. The lower chamber was filled with X‐VIVO containing <t>CCL3</t> (200 ng mL −1 ) in (j) . Data are represented as mean ± SEM and were analysed by the unpaired Student's t‐ test (b, f, i, j) or one‐way ANOVA (g, h) . * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are pooled from three independent experiments.
Nci 11292 Human Nsclc Xenograft Model, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nci-11292 human nsclc xenograft model/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
nci-11292 human nsclc xenograft model - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
models for se coordination modes (11292 or 2) to the mof zr-cluster  (CrystalMaker)
90
CrystalMaker models for se coordination modes (11292 or 2) to the mof zr-cluster
CD177 promotes T‐cell transendothelial migration. (a) Human colorectal cancer (CRC) specimens were stained with DAPI (blue), PEACM‐1 (green), CD4 (white), FOXP3 (red) and CD177 (yellow), respectively. The scale bar (50 μm) is placed at the left corner of each image. (b) CD177 + Treg cells in peripheral blood mononuclear cells from CRC patients ( n = 12) and healthy volunteers ( n = 6). (c) Diagram of CD177‐EGFP in pHR plasmid. (d) Model of iTreg cells induction, transfection, selection and then expanded for 2 days before the assay. (e) Diagram of the transendothelial migration assay. HUVECs were seeded on the upper surface of the transwell insert and iTreg cells were placed in the upper chamber filled with X‐VIVO. The lower chamber was filled with X‐VIVO containing CCL19 and CCL22 (200 ng mL −1 ). (f) Migration rate of OE iTreg cells and EV iTreg cells after 4 h. OE: CD177‐overexpression; EV: empty vector control. (g) Migration rate of OE iTreg cells at 4 h under different treatments. NC, negative control; α177, anti‐CD177; αPE, anti‐PECAM‐1. (h) Migration rate of EV iTreg cells after 4 h under different treatments. (i) PD‐1 expression of OE iTreg cells and EV iTreg cells. (j) Migration rate of OE CD8 + T cells and EV CD8 + T cells at 4 h. The lower chamber was filled with X‐VIVO containing <t>CCL3</t> (200 ng mL −1 ) in (j) . Data are represented as mean ± SEM and were analysed by the unpaired Student's t‐ test (b, f, i, j) or one‐way ANOVA (g, h) . * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are pooled from three independent experiments.
Models For Se Coordination Modes (11292 Or 2) To The Mof Zr Cluster, supplied by CrystalMaker, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/models for se coordination modes (11292 or 2) to the mof zr-cluster/product/CrystalMaker
Average 90 stars, based on 1 article reviews
models for se coordination modes (11292 or 2) to the mof zr-cluster - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
actinobacteria  (ATCC)
92
ATCC actinobacteria
CD177 promotes T‐cell transendothelial migration. (a) Human colorectal cancer (CRC) specimens were stained with DAPI (blue), PEACM‐1 (green), CD4 (white), FOXP3 (red) and CD177 (yellow), respectively. The scale bar (50 μm) is placed at the left corner of each image. (b) CD177 + Treg cells in peripheral blood mononuclear cells from CRC patients ( n = 12) and healthy volunteers ( n = 6). (c) Diagram of CD177‐EGFP in pHR plasmid. (d) Model of iTreg cells induction, transfection, selection and then expanded for 2 days before the assay. (e) Diagram of the transendothelial migration assay. HUVECs were seeded on the upper surface of the transwell insert and iTreg cells were placed in the upper chamber filled with X‐VIVO. The lower chamber was filled with X‐VIVO containing CCL19 and CCL22 (200 ng mL −1 ). (f) Migration rate of OE iTreg cells and EV iTreg cells after 4 h. OE: CD177‐overexpression; EV: empty vector control. (g) Migration rate of OE iTreg cells at 4 h under different treatments. NC, negative control; α177, anti‐CD177; αPE, anti‐PECAM‐1. (h) Migration rate of EV iTreg cells after 4 h under different treatments. (i) PD‐1 expression of OE iTreg cells and EV iTreg cells. (j) Migration rate of OE CD8 + T cells and EV CD8 + T cells at 4 h. The lower chamber was filled with X‐VIVO containing <t>CCL3</t> (200 ng mL −1 ) in (j) . Data are represented as mean ± SEM and were analysed by the unpaired Student's t‐ test (b, f, i, j) or one‐way ANOVA (g, h) . * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are pooled from three independent experiments.
Actinobacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actinobacteria/product/ATCC
Average 92 stars, based on 1 article reviews
actinobacteria - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier

Image Search Results


CD177 promotes T‐cell transendothelial migration. (a) Human colorectal cancer (CRC) specimens were stained with DAPI (blue), PEACM‐1 (green), CD4 (white), FOXP3 (red) and CD177 (yellow), respectively. The scale bar (50 μm) is placed at the left corner of each image. (b) CD177 + Treg cells in peripheral blood mononuclear cells from CRC patients ( n = 12) and healthy volunteers ( n = 6). (c) Diagram of CD177‐EGFP in pHR plasmid. (d) Model of iTreg cells induction, transfection, selection and then expanded for 2 days before the assay. (e) Diagram of the transendothelial migration assay. HUVECs were seeded on the upper surface of the transwell insert and iTreg cells were placed in the upper chamber filled with X‐VIVO. The lower chamber was filled with X‐VIVO containing CCL19 and CCL22 (200 ng mL −1 ). (f) Migration rate of OE iTreg cells and EV iTreg cells after 4 h. OE: CD177‐overexpression; EV: empty vector control. (g) Migration rate of OE iTreg cells at 4 h under different treatments. NC, negative control; α177, anti‐CD177; αPE, anti‐PECAM‐1. (h) Migration rate of EV iTreg cells after 4 h under different treatments. (i) PD‐1 expression of OE iTreg cells and EV iTreg cells. (j) Migration rate of OE CD8 + T cells and EV CD8 + T cells at 4 h. The lower chamber was filled with X‐VIVO containing CCL3 (200 ng mL −1 ) in (j) . Data are represented as mean ± SEM and were analysed by the unpaired Student's t‐ test (b, f, i, j) or one‐way ANOVA (g, h) . * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are pooled from three independent experiments.

Journal: Clinical & Translational Immunology

Article Title: CD177 drives the transendothelial migration of Treg cells enriched in human colorectal cancer

doi: 10.1002/cti2.1506

Figure Lengend Snippet: CD177 promotes T‐cell transendothelial migration. (a) Human colorectal cancer (CRC) specimens were stained with DAPI (blue), PEACM‐1 (green), CD4 (white), FOXP3 (red) and CD177 (yellow), respectively. The scale bar (50 μm) is placed at the left corner of each image. (b) CD177 + Treg cells in peripheral blood mononuclear cells from CRC patients ( n = 12) and healthy volunteers ( n = 6). (c) Diagram of CD177‐EGFP in pHR plasmid. (d) Model of iTreg cells induction, transfection, selection and then expanded for 2 days before the assay. (e) Diagram of the transendothelial migration assay. HUVECs were seeded on the upper surface of the transwell insert and iTreg cells were placed in the upper chamber filled with X‐VIVO. The lower chamber was filled with X‐VIVO containing CCL19 and CCL22 (200 ng mL −1 ). (f) Migration rate of OE iTreg cells and EV iTreg cells after 4 h. OE: CD177‐overexpression; EV: empty vector control. (g) Migration rate of OE iTreg cells at 4 h under different treatments. NC, negative control; α177, anti‐CD177; αPE, anti‐PECAM‐1. (h) Migration rate of EV iTreg cells after 4 h under different treatments. (i) PD‐1 expression of OE iTreg cells and EV iTreg cells. (j) Migration rate of OE CD8 + T cells and EV CD8 + T cells at 4 h. The lower chamber was filled with X‐VIVO containing CCL3 (200 ng mL −1 ) in (j) . Data are represented as mean ± SEM and were analysed by the unpaired Student's t‐ test (b, f, i, j) or one‐way ANOVA (g, h) . * P < 0.05, ** P < 0.01, **** P < 0.0001. Data are pooled from three independent experiments.

Article Snippet: Next, 600 μL X‐VIVO medium containing CCL19 and CCL21 (200 ng mL −1 , Cat#300‐29B and 300‐36A, Peprotech) or CCL3 (200 ng mL −1 , Cat#11292‐H08Y, SinoBiological) were placed in the lower chamber.

Techniques: Migration, Staining, Plasmid Preparation, Transfection, Selection, Over Expression, Negative Control, Expressing